Analysis Note

Positive ControlEndothelial cells or tonsil tissue

Application

Cell Inhibition Assays (see comments)r>Flow Cytometry (see comments)r>Frozen Sections (1:50-1:100)r>Immunoblotting (1:200-1:400)r>Immunofluorescence (see comments)r>Immunoprecipitation (10 µl/mg protein lysate)r>Paraffin Sections (not recommended)

General description

Purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing Balb/c splenocytes with p3-NS-1/Ag4-1 (NS1) mouse myeloma cells (see application references). Recognizes the ~85-115 kDa ICAM-1 protein.

This Anti-ICAM-1 (Ab-2) Mouse mAb (W-CAM-1) is validated for use in Cell Inhibition Assays, FC, Frozen Sections, Immunoblotting, IF, IP, Paraffin Sections for the detection of ICAM-1 (Ab-2).

Recognizes the ~85-115 kDa ICAM-1 protein in endothelial cells and tonsil tissue.

Immunogen

Human

Raji cells

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

This antibody will block aggregation of cell lines mediated by ICAM-1 and blocks homotypic binding of purified populations of activated T- and B-lymphocytes and aggregation of mixed T- and B-cell blasts. Will inhibit T-cell adhesion to normal human endothelial cells. Activation induced by cell-cell contact (mixed lymphocyte reaction, T cell mediated B-cell activation) is significantly inhibited. This antibody will also block elements of both effector arms of the immune system (cytotoxic cell function and Ig production). This antibody has been reported to work for immunofluorescence and flow cytometry studies. Antibody should be titrated for optimal results in individual systems.

Takami, S., et al. 1998. Circ.97, 721.Craig, A.G., et al. 1997. Infect. Immun.65, 4580.DeGraba, T.J. 1997. Neurology49, S15.Elangbam, C.S., et al. 1997. Vet. Pathol. 34, 61.McCormick, C.J., et al. 1997. J. Clin. Invest.15, 2521.Acevedo, A., et al. 1993. Am. J. Pathol.143, 774.De Fougerolles, A.R., et al. 1993. J. Exp. Med.177, 1187.Fawcett, J., et al. 1992. Nature360, 481.Vazeux, R. et al. 1992. Nature360, 485.Boyd, A.W., et al. 1989. Blood73, 1896.

Packaging

100 µg in Plastic ampoule

Please refer to vial label for lot-specific concentration.

Physical form

In 10 mM PBS, 0.2% BSA, pH 7.4.

Reconstitution

Following initial use, aliquot and freeze (-20°C) for long-term storage if desired. Avoid freeze/thaw.

Warning

Toxicity: Standard Handling (A)